endothelial cell basal medium huvec  (PromoCell)

Journal: Journal of Extracellular Vesicles

Article Title: The Fatty Acid Transporter CD36 Mediates Uptake, Biodistribution, and Cardioprotection by Small Extracellular Vesicles From HEK293 Cells

doi: 10.1002/jev2.70254

Figure Lengend Snippet: Dose‐response and time‐response uptake of HEK293‐sEVs in human endothelial cells and cardiomyocytes. (A–C) Human coronary artery and coronary microvascular endothelial cells (HCAEC and HCMEC respectively) and human umbilical vein endothelial cells (HUVEC) were treated for 4 h with increasing doses of HEK293‐sEVs. A linear dose‐response was revealed in all endothelial cells, as a 10‐fold increase of sEVs dose results in a 10‐fold increase of cellular uptake (Nluc Nanoluciferase activity). (D–F) sEVs rapidly internalise endothelial cells ((D) HCAEC, (E) HCMEC, and (F) HUVEC) within the first hour of incubation reaching the highest intracellular localisation after 4 h, corresponding to higher Nluc activity. A reduction in luciferase activity after 24 h is observed in all endothelial cells, reflective of the reduced amount of sEVs intracellularly. (G) Uptake of HEK293‐sEVs in adult rat cardiomyocytes is slow and time‐dependent, and high Nluc activity is recorded only after 24 h of sEVs incubation. Data shown as mean ± SEM ( n = 4). * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: HUVEC (Human Umbilical Vein Endothelial Cells) were cultured in endothelial cell basal medium 2 (PromoCell, C‐22011) supplemented with the supplement mix (PromoCell, C‐39216).

Techniques: Activity Assay, Incubation, Luciferase

Journal: NPJ Microgravity

Article Title: Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flight

doi: 10.1038/s41526-024-00456-7

Figure Lengend Snippet: Representative images ( a ) before and ( b ) after floating THP-1 cells adhere to the HUVEC layer (arrow). Scale bar: 50 µm. c The number of newly adhering THP-1 cells to the HUVEC layer within each parabola of the partial gravity phases (0.16 g and 0.38 g ) and in 1 g assessed by video analysis (cell number/FOV (field of view)). Bar charts represent mean values ± SD and symbols represent number of cells/FOV. d Total number of THP-1 cells adhering to HUVEC layer assessed by confocal microscopic assessment (cell number/FOV). Inflight fixation of samples was performed after P8 and numbers were compared to baseline collection on ground (1 g ). Boxes indicate median and interquartile range; whiskers represent minimum and maximum (three FOV/slide; two slides per condition for 0.16 g and 1 g and one slide per condition for 0.38 g ). * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: HUVECs (cat. no. 00191027, Lonza, Basel, Switzerland) were seeded in HUVEC full medium (cat. no. C-22215 supplemented with C-39215, PromoCell, Heidelberg, Germany) into 0.4 mm ibidi µ-slides (cat. no. 80176, ibidi, Graefelfing, Germany) in a cell density of 0.4–0.5 × 10 6 cells in 200 µl and allowed to attach for 24 h. When cells were attached, slides were installed into the ibidi pump system (ibidi, Graefelfing, Germany) and HUVECs were cultivated under constant flow (shear stress 5 dyn/cm 2 ) for additional seven days, enabling to produce a glycocalyx (Supplementary Fig. ).

Techniques: